WHAT IS PCR?

Polymerase chain reaction (PCR), sometimes called “molecular photocopying,” is a common laboratory technique used to amplify a segment of DNA of interest or produce lots of copies. In other words, PCR enables you to produce millions of copies of a specific DNA sequence from an initially small sample – sometimes even a single copy. In PCR, for allowing many copies of the target DNA to be produced, the reaction is repeatedly cycled through a series of temperature changes.

WHAT IS PCR USED FOR?

Once amplified, the DNA produced by PCR can be used in many different laboratory procedures. PCR method, which ia generally used in medical and biological research, is frequently used in medical and genetic research too. In addition to them, PCR is useful to diognosis of genetic diseases.

Also, PCR allows for rapid and highly specific diagnosis of infectious diseases, including those caused by bacteria or viruses such as Covid-19 SARS-CoV-2   .

PCR TEST FOR COVID-19

Over the course of the current Covid-19 crisis, the importance of reliable, accessible testing to screen for the disease has become increasingly apparent. Tests fort he detection of  Covid-19 SARS-CoV-2 are PCR test and antigen tests, also for detecting immunity we use serelogical methods and Antibody tests.

PCR tests are used to directly detect the presence of an antigen, rather than the presence of the body’s immune response, or antibodies. By detecting viral RNA, which will be present in the body before antibodies form or symptoms of the disease are present, the tests can tell whether or not someone has the virus very early on. Real time reverse transcriptase polymerase is the gold standart method for confirming the diagnosis of Covid-19 SARS-CoV-2 ‘s RNA in the respiratery system.

HOW TO DO PCR TEST ?

Covid-19 PCR test performed by health care professionals within the appropriate precautions. Professionals use a specialized swab to collect mucus from your nose or throat and put the swab in a sterile container  for transport to a lab. This swaps could stand 24 hours without deterioration in this containers, so if necssary they can transportded to the labratuars. In labratuar a lab technician uses  primers and probes, and a otomatik machine to conduct several controlled heating and cooling cycles to convert the virus’s RNA into DNA, and then make millions of copies of the DNA. At the end of this processes, if the DNA combines with the probes, results would be “positive”

IS PCR TEST SAFE?

Covid-19 PCR test in the main diognostic test and it is the safest one. Thanks to the Pcr test, carriers show no symptoms of the diesease can be found and necsseary measures can be taken. To increase the reliability of the PCR test, sampling and proper handling of samples are important